rabbit anti-hpv16-l1, anti-hpv16-e6, anti-hpv16-e7  (R&D Systems)

Journal: Scientific Reports

Article Title: Attenuated Salmonella carrying plasmid co-expressing HPV16 L1 and siRNA-E6 for cervical cancer therapy

doi: 10.1038/s41598-021-99425-3

Figure Lengend Snippet: The pcDNA3.1-HPV16-L1 plasmid was successfully constructed and HPV16-L1 was identified to express in BHK cells. ( A ) The map of pcDNA3.1-HPV16-L1. ( B ) Identification of pcDNA3.1-HPV16-L1 by restriction enzyme digestion. (M1: DL2 000; lane 12: plasmid digested by HindIII and KpnI; M2: DL15000). ( C ) SDS PAGE analysis of HPV16-L1 protein expressed in BHK cells (M: protein marker, lane 1: control, lane 2: expressed protein). ( D ) Western blotting analysis of HPV16-L1 protein expressed in BHK cells (M: protein marker; lane 1: control; lane 2: expressed protein). ( E ) Immunocytochemistry analysis of HPV16-L1 protein expressed in BHK(× 400). ( F ) Electron microscropy detection of HPV16-L1 expressed in BHK cells.

Article Snippet: Rabbit anti-HPV16-L1, anti-HPV16-E6, anti-HPV16-E7 (R&D, USA), Rabbit anti-P53, anti-Bax, anti-Caspase9, anti-Caspase3, anti-cleaved-Caspase3 and anti-β-actin (Affinity, China) were used as the primary antibodies.

Techniques: Plasmid Preparation, Construct, SDS Page, Marker, Western Blot, Immunocytochemistry

Journal: Scientific Reports

Article Title: Attenuated Salmonella carrying plasmid co-expressing HPV16 L1 and siRNA-E6 for cervical cancer therapy

doi: 10.1038/s41598-021-99425-3

Figure Lengend Snippet: After immune with attenuated Salmonella carrying pcDNA3.1-HPV16-L1, antibodies were produced and cytokines were increased in mice. ( A ) ELISA detection of anti-HPV16-L1 antibody after vaccinated 1 time. *P < 0.05, **P < 0.01, vs the 0d group. ( B ) ELISA detection of IL-2 and IFN-γ after vaccinated 3 times. *P < 0.05, **P < 0.01, vs the 0d group. ( C , D ) ELISA detection of anti-HPV16-L1 antibody after vaccinated 3 times. **P < 0.01, vs the control group. ( E , F ) ELISA detection of IL-2 and IFN-γ after vaccinated 3 times. *P < 0.05, **P < 0.01, vs the control group.

Article Snippet: Rabbit anti-HPV16-L1, anti-HPV16-E6, anti-HPV16-E7 (R&D, USA), Rabbit anti-P53, anti-Bax, anti-Caspase9, anti-Caspase3, anti-cleaved-Caspase3 and anti-β-actin (Affinity, China) were used as the primary antibodies.

Techniques: Produced, Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Attenuated Salmonella carrying plasmid co-expressing HPV16 L1 and siRNA-E6 for cervical cancer therapy

doi: 10.1038/s41598-021-99425-3

Figure Lengend Snippet: After transfection with pGC-siE6A or pGC-siE7 plasmid, the expression of HPV-E6/E7 protein was decreased in Siha cells. ( A ) The map of pGC-siRNA. ( B ) Semi-quantitative RT-PCR analysis of HPV16-E6/E7 mRNA in Siha cells transfected with pGC-siRNA plasmid. ( C , D ) Western blotting analysis of HPV16-E6 and HPV16-E7 protein in Siha cells transfected with pGC-siRNA plasmid. ( E ) Transfection efficiency determined by fluorescence microscope. ( F , G ) Immunofluorescence analysis for HPV16-E6/E7 protein in Siha cells transfected with pGC-siRNA plasmids (× 600).

Article Snippet: Rabbit anti-HPV16-L1, anti-HPV16-E6, anti-HPV16-E7 (R&D, USA), Rabbit anti-P53, anti-Bax, anti-Caspase9, anti-Caspase3, anti-cleaved-Caspase3 and anti-β-actin (Affinity, China) were used as the primary antibodies.

Techniques: Transfection, Plasmid Preparation, Expressing, Quantitative RT-PCR, Western Blot, Fluorescence, Microscopy, Immunofluorescence

Journal: Scientific Reports

Article Title: Attenuated Salmonella carrying plasmid co-expressing HPV16 L1 and siRNA-E6 for cervical cancer therapy

doi: 10.1038/s41598-021-99425-3

Figure Lengend Snippet: pCG-siE6 can inhibit the growth of the xenograft tumors in vivo. ( A ) Growth of Siha xenografts treated with pGC-siE6 (n = 5). Each treatment with the plasmid is shown an arrow. **P < 0.01, vs the two control groups. ( B ) Semi-quantitative RT-PCR analysis of HPV16-E6/E7 mRNA in Siha xenografts treated with pGC-siE6 plasmids. **P < 0.01, vs the two control groups. ( C ) HE assay for Siha xenografts treated with pGC-siE6 plasmids (× 400). ( D ) Fluorescence immunohistochemical analysis of HPV16-E6/E7 protein expression in Siha xenografts treated with plasmids (× 600).

Article Snippet: Rabbit anti-HPV16-L1, anti-HPV16-E6, anti-HPV16-E7 (R&D, USA), Rabbit anti-P53, anti-Bax, anti-Caspase9, anti-Caspase3, anti-cleaved-Caspase3 and anti-β-actin (Affinity, China) were used as the primary antibodies.

Techniques: In Vivo, Plasmid Preparation, Quantitative RT-PCR, Fluorescence, Immunohistochemical staining, Expressing

Journal: Scientific Reports

Article Title: Attenuated Salmonella carrying plasmid co-expressing HPV16 L1 and siRNA-E6 for cervical cancer therapy

doi: 10.1038/s41598-021-99425-3

Figure Lengend Snippet: The mechanism of phoP/phoQ(pcDNA3.1-L1-siE6) inhibiting the growth of xenograft tumors in vivo. ( A ) ELISA analysis of anti-HPV16-L1 antibody in serum after treated with phoP/phoQ. *P < 0.05, vs the control group. ( B , C ) ELISA anal-ysis of IL-2 and IFN-γ in serum after treated with phoP/phoQ. *P < 0.05, vs the control group. ( D – F ) Immunohistochemical analysis of HPV-L1/E6/E7 protein expression in Siha xenografts treated with phoP/phoQ (× 400).

Article Snippet: Rabbit anti-HPV16-L1, anti-HPV16-E6, anti-HPV16-E7 (R&D, USA), Rabbit anti-P53, anti-Bax, anti-Caspase9, anti-Caspase3, anti-cleaved-Caspase3 and anti-β-actin (Affinity, China) were used as the primary antibodies.

Techniques: In Vivo, Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Expressing

Journal: Scientific Reports

Article Title: Attenuated Salmonella carrying plasmid co-expressing HPV16 L1 and siRNA-E6 for cervical cancer therapy

doi: 10.1038/s41598-021-99425-3

Figure Lengend Snippet: The sequences of RT-PCR primers.

Article Snippet: Rabbit anti-HPV16-L1, anti-HPV16-E6, anti-HPV16-E7 (R&D, USA), Rabbit anti-P53, anti-Bax, anti-Caspase9, anti-Caspase3, anti-cleaved-Caspase3 and anti-β-actin (Affinity, China) were used as the primary antibodies.

Techniques:

Journal: Scientific Reports

Article Title: Attenuated Salmonella carrying plasmid co-expressing HPV16 L1 and siRNA-E6 for cervical cancer therapy

doi: 10.1038/s41598-021-99425-3

Figure Lengend Snippet: The pcDNA3.1-HPV16-L1 plasmid was successfully constructed and HPV16-L1 was identified to express in BHK cells. ( A ) The map of pcDNA3.1-HPV16-L1. ( B ) Identification of pcDNA3.1-HPV16-L1 by restriction enzyme digestion. (M1: DL2 000; lane 12: plasmid digested by HindIII and KpnI; M2: DL15000). ( C ) SDS PAGE analysis of HPV16-L1 protein expressed in BHK cells (M: protein marker, lane 1: control, lane 2: expressed protein). ( D ) Western blotting analysis of HPV16-L1 protein expressed in BHK cells (M: protein marker; lane 1: control; lane 2: expressed protein). ( E ) Immunocytochemistry analysis of HPV16-L1 protein expressed in BHK(× 400). ( F ) Electron microscropy detection of HPV16-L1 expressed in BHK cells.

Article Snippet: Rabbit anti-HPV16-L1, anti-HPV16-E6, anti-HPV16-E7 (R&D, USA), Rabbit anti-P53, anti-Bax, anti-Caspase9, anti-Caspase3, anti-cleaved-Caspase3 and anti-β-actin (Affinity, China) were used as the primary antibodies.

Techniques: Plasmid Preparation, Construct, SDS Page, Marker, Western Blot, Immunocytochemistry

Journal: Scientific Reports

Article Title: Attenuated Salmonella carrying plasmid co-expressing HPV16 L1 and siRNA-E6 for cervical cancer therapy

doi: 10.1038/s41598-021-99425-3

Figure Lengend Snippet: After immune with attenuated Salmonella carrying pcDNA3.1-HPV16-L1, antibodies were produced and cytokines were increased in mice. ( A ) ELISA detection of anti-HPV16-L1 antibody after vaccinated 1 time. *P < 0.05, **P < 0.01, vs the 0d group. ( B ) ELISA detection of IL-2 and IFN-γ after vaccinated 3 times. *P < 0.05, **P < 0.01, vs the 0d group. ( C , D ) ELISA detection of anti-HPV16-L1 antibody after vaccinated 3 times. **P < 0.01, vs the control group. ( E , F ) ELISA detection of IL-2 and IFN-γ after vaccinated 3 times. *P < 0.05, **P < 0.01, vs the control group.

Article Snippet: Rabbit anti-HPV16-L1, anti-HPV16-E6, anti-HPV16-E7 (R&D, USA), Rabbit anti-P53, anti-Bax, anti-Caspase9, anti-Caspase3, anti-cleaved-Caspase3 and anti-β-actin (Affinity, China) were used as the primary antibodies.

Techniques: Produced, Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Attenuated Salmonella carrying plasmid co-expressing HPV16 L1 and siRNA-E6 for cervical cancer therapy

doi: 10.1038/s41598-021-99425-3

Figure Lengend Snippet: After transfection with pGC-siE6A or pGC-siE7 plasmid, the expression of HPV-E6/E7 protein was decreased in Siha cells. ( A ) The map of pGC-siRNA. ( B ) Semi-quantitative RT-PCR analysis of HPV16-E6/E7 mRNA in Siha cells transfected with pGC-siRNA plasmid. ( C , D ) Western blotting analysis of HPV16-E6 and HPV16-E7 protein in Siha cells transfected with pGC-siRNA plasmid. ( E ) Transfection efficiency determined by fluorescence microscope. ( F , G ) Immunofluorescence analysis for HPV16-E6/E7 protein in Siha cells transfected with pGC-siRNA plasmids (× 600).

Article Snippet: Rabbit anti-HPV16-L1, anti-HPV16-E6, anti-HPV16-E7 (R&D, USA), Rabbit anti-P53, anti-Bax, anti-Caspase9, anti-Caspase3, anti-cleaved-Caspase3 and anti-β-actin (Affinity, China) were used as the primary antibodies.

Techniques: Transfection, Plasmid Preparation, Expressing, Quantitative RT-PCR, Western Blot, Fluorescence, Microscopy, Immunofluorescence

Journal: Scientific Reports

Article Title: Attenuated Salmonella carrying plasmid co-expressing HPV16 L1 and siRNA-E6 for cervical cancer therapy

doi: 10.1038/s41598-021-99425-3

Figure Lengend Snippet: pCG-siE6 can inhibit the growth of the xenograft tumors in vivo. ( A ) Growth of Siha xenografts treated with pGC-siE6 (n = 5). Each treatment with the plasmid is shown an arrow. **P < 0.01, vs the two control groups. ( B ) Semi-quantitative RT-PCR analysis of HPV16-E6/E7 mRNA in Siha xenografts treated with pGC-siE6 plasmids. **P < 0.01, vs the two control groups. ( C ) HE assay for Siha xenografts treated with pGC-siE6 plasmids (× 400). ( D ) Fluorescence immunohistochemical analysis of HPV16-E6/E7 protein expression in Siha xenografts treated with plasmids (× 600).

Article Snippet: Rabbit anti-HPV16-L1, anti-HPV16-E6, anti-HPV16-E7 (R&D, USA), Rabbit anti-P53, anti-Bax, anti-Caspase9, anti-Caspase3, anti-cleaved-Caspase3 and anti-β-actin (Affinity, China) were used as the primary antibodies.

Techniques: In Vivo, Plasmid Preparation, Quantitative RT-PCR, Fluorescence, Immunohistochemical staining, Expressing

Journal: Scientific Reports

Article Title: Attenuated Salmonella carrying plasmid co-expressing HPV16 L1 and siRNA-E6 for cervical cancer therapy

doi: 10.1038/s41598-021-99425-3

Figure Lengend Snippet: The mechanism of phoP/phoQ(pcDNA3.1-L1-siE6) inhibiting the growth of xenograft tumors in vivo. ( A ) ELISA analysis of anti-HPV16-L1 antibody in serum after treated with phoP/phoQ. *P < 0.05, vs the control group. ( B , C ) ELISA anal-ysis of IL-2 and IFN-γ in serum after treated with phoP/phoQ. *P < 0.05, vs the control group. ( D – F ) Immunohistochemical analysis of HPV-L1/E6/E7 protein expression in Siha xenografts treated with phoP/phoQ (× 400).

Article Snippet: Rabbit anti-HPV16-L1, anti-HPV16-E6, anti-HPV16-E7 (R&D, USA), Rabbit anti-P53, anti-Bax, anti-Caspase9, anti-Caspase3, anti-cleaved-Caspase3 and anti-β-actin (Affinity, China) were used as the primary antibodies.

Techniques: In Vivo, Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Expressing

Journal: Scientific Reports

Article Title: Attenuated Salmonella carrying plasmid co-expressing HPV16 L1 and siRNA-E6 for cervical cancer therapy

doi: 10.1038/s41598-021-99425-3

Figure Lengend Snippet: The sequences of RT-PCR primers.

Article Snippet: Rabbit anti-HPV16-L1, anti-HPV16-E6, anti-HPV16-E7 (R&D, USA), Rabbit anti-P53, anti-Bax, anti-Caspase9, anti-Caspase3, anti-cleaved-Caspase3 and anti-β-actin (Affinity, China) were used as the primary antibodies.

Techniques:

Journal: Cell reports

Article Title: An Immunocompetent Mouse Model of HPV16(+) Head and Neck Squamous Cell Carcinoma

doi: 10.1016/j.celrep.2019.10.005

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit anti-HPV16 E6 (1:1000) , Gene Tex , Cat# GTX132686.

Techniques: Virus, Recombinant, SYBR Green Assay, Luciferase, Gene Expression, Plasmid Preparation, Software